Question, I have been loading the 727-200F while on the tarmac with the little animated loader that you see putting the containers in the aircraft, do I still have to use ACE before I load the aircraft, or can I skip ACE and just load the plane with the animated loader and still get the weight that I want?

Thanks for the clarification  :)

You only need ACE when you want to change the weight of the individual Unit Load Device [ULD]. If you have the weight set at the pounds (or kilograms) you use for each flight then just skip and use the loader to put on the number of ULD's that equal your cargo weight.